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hcd45 microbeads (Miltenyi Biotec)

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Miltenyi Biotec hcd45 microbeads

a Schematic of wildtype and large deletion cassettes used to establish the LoD of large deletions, and representation of clonal expansion of cells harbouring a large deletion (orange) mediated genotoxic aberration amongst wildtype cells (blue). Dark and light grey bar indicates CCR5 and reference sequences, respectively. Red bar indicates flag sequence used to fuse assay and reference sequences. b Correlation of observed against the expected percentage of large deletions. Vertical black dotted line represents the limit of detection. Solid and dotted red line represents line of regression with 95% confidence interval, respectively. R 2 = 0.997. Calculated with linear regression analysis in GraphPad. Data shown as mean ± s.d. of n = 4 technical replicates. c CLEAR-time dPCR summaries of Cas9-edited HSPCs targeting various genes at different timepoints. The BTK edited HSPCs were also transduced with AAV6 encoding GFP. All editing was normalised against unedited mock electroporated HSPCs. Data are shown as mean ± s.d. d CLEAR-time dPCR summary of SH2D1A edited T cells with Cas9, Cas12, and TALENs at 3 days post-editing. Data are shown as mean ± s.d. e CLEAR-time dPCR summary of on-target CCR5 edited with decreasing concentrations of Cas9 at 3 h and 3 days post-editing. Data are shown as mean ± s.d. f DSB and indels quantification at three known off-targets targeting CCR5 with decreasing concentrations of Cas9 at 3 h and 3 days post-editing. Data are shown as mean ± s.d. g CLEAR-time dPCR on XIAP edited HSPCs pre-transplant and 16 weeks post-transplant. Data are shown as mean ± s.d. n = 4 mice for each treatment group. h Integration frequency in 16-week post-transplant XIAP edited and AAV-transduced <t>hCD45</t> cells by dPCR and flow cytometry. Flow cytometry data represent n = 1 per mouse, dPCR data shown as mean ± s.d. of n = 3 technical replicates. i CLEAR-time dPCR normalised ICE analysis of pre-transplant XIAP edited HSPCs and post-transplant XIAP edited hCD45 cells. All data represents n = 3 technical replicates unless stated otherwise. d – f Two-way ANOVA with Tukey post-hoc test. n.s.= no statistical significance, **** p < 0.0001. Source data are provided as a Source Data file.

Hcd45 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 466 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hcd45+microbeads/CD45+MicroBeads%2C+human/pmc12583642-485-7-9
Average 96 stars, based on 466 article reviews

hcd45 microbeads - by Bioz Stars, 2026-10

96/100 stars

Images

1) Product Images from "Unveiling the cut-and-repair cycle of designer nucleases in human stem and T cells via CLEAR-time dPCR"

Article Title: Unveiling the cut-and-repair cycle of designer nucleases in human stem and T cells via CLEAR-time dPCR

Journal: Nature Communications

doi: 10.1038/s41467-025-65182-4

Figure Legend Snippet: a Schematic of wildtype and large deletion cassettes used to establish the LoD of large deletions, and representation of clonal expansion of cells harbouring a large deletion (orange) mediated genotoxic aberration amongst wildtype cells (blue). Dark and light grey bar indicates CCR5 and reference sequences, respectively. Red bar indicates flag sequence used to fuse assay and reference sequences. b Correlation of observed against the expected percentage of large deletions. Vertical black dotted line represents the limit of detection. Solid and dotted red line represents line of regression with 95% confidence interval, respectively. R 2 = 0.997. Calculated with linear regression analysis in GraphPad. Data shown as mean ± s.d. of n = 4 technical replicates. c CLEAR-time dPCR summaries of Cas9-edited HSPCs targeting various genes at different timepoints. The BTK edited HSPCs were also transduced with AAV6 encoding GFP. All editing was normalised against unedited mock electroporated HSPCs. Data are shown as mean ± s.d. d CLEAR-time dPCR summary of SH2D1A edited T cells with Cas9, Cas12, and TALENs at 3 days post-editing. Data are shown as mean ± s.d. e CLEAR-time dPCR summary of on-target CCR5 edited with decreasing concentrations of Cas9 at 3 h and 3 days post-editing. Data are shown as mean ± s.d. f DSB and indels quantification at three known off-targets targeting CCR5 with decreasing concentrations of Cas9 at 3 h and 3 days post-editing. Data are shown as mean ± s.d. g CLEAR-time dPCR on XIAP edited HSPCs pre-transplant and 16 weeks post-transplant. Data are shown as mean ± s.d. n = 4 mice for each treatment group. h Integration frequency in 16-week post-transplant XIAP edited and AAV-transduced hCD45 cells by dPCR and flow cytometry. Flow cytometry data represent n = 1 per mouse, dPCR data shown as mean ± s.d. of n = 3 technical replicates. i CLEAR-time dPCR normalised ICE analysis of pre-transplant XIAP edited HSPCs and post-transplant XIAP edited hCD45 cells. All data represents n = 3 technical replicates unless stated otherwise. d – f Two-way ANOVA with Tukey post-hoc test. n.s.= no statistical significance, **** p < 0.0001. Source data are provided as a Source Data file.

Techniques Used: Sequencing, Transduction, TALENs, Flow Cytometry

Magnetic Cell Separation:

Article Title: Quinacrine-CASIN combination overcomes chemoresistance in human acute lymphoid leukemia
Article Snippet: Non-adherent cells present in supernatant medium were washed with PBS and passed through a 15 μm filter (pluriSelect Life Science, Leipzig, Germany). .. ALL cells were separated from MSCs by magnetic cell separation using hCD45 microbeads (Miltenyi Biotec, Auburn CA). ..

Isolation:

Article Title: Improving Gene Editing Outcomes in Human Hematopoietic Stem and Progenitor Cells by Temporal Control of DNA Repair
Article Snippet: .. The human cells from mouse BM were isolated using hCD45+ microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany), gDNA was extracted, and the levels of gene editing in human cells were determined by HTS, as described earlier. ..

Article Title: Improving Nuclease-Mediated Gene Editing Outcomes in Human Hematopoietic Stem Cells
Article Snippet: .. The human cells from mouse BM were isolated using hCD45+ microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany), gDNA was extracted, and the levels of gene editing in human cells were determined by HTS, as described earlier. ..

Image Search Results

Journal: Nature Communications

Article Title: Unveiling the cut-and-repair cycle of designer nucleases in human stem and T cells via CLEAR-time dPCR

doi: 10.1038/s41467-025-65182-4

Figure Lengend Snippet: a Schematic of wildtype and large deletion cassettes used to establish the LoD of large deletions, and representation of clonal expansion of cells harbouring a large deletion (orange) mediated genotoxic aberration amongst wildtype cells (blue). Dark and light grey bar indicates CCR5 and reference sequences, respectively. Red bar indicates flag sequence used to fuse assay and reference sequences. b Correlation of observed against the expected percentage of large deletions. Vertical black dotted line represents the limit of detection. Solid and dotted red line represents line of regression with 95% confidence interval, respectively. R 2 = 0.997. Calculated with linear regression analysis in GraphPad. Data shown as mean ± s.d. of n = 4 technical replicates. c CLEAR-time dPCR summaries of Cas9-edited HSPCs targeting various genes at different timepoints. The BTK edited HSPCs were also transduced with AAV6 encoding GFP. All editing was normalised against unedited mock electroporated HSPCs. Data are shown as mean ± s.d. d CLEAR-time dPCR summary of SH2D1A edited T cells with Cas9, Cas12, and TALENs at 3 days post-editing. Data are shown as mean ± s.d. e CLEAR-time dPCR summary of on-target CCR5 edited with decreasing concentrations of Cas9 at 3 h and 3 days post-editing. Data are shown as mean ± s.d. f DSB and indels quantification at three known off-targets targeting CCR5 with decreasing concentrations of Cas9 at 3 h and 3 days post-editing. Data are shown as mean ± s.d. g CLEAR-time dPCR on XIAP edited HSPCs pre-transplant and 16 weeks post-transplant. Data are shown as mean ± s.d. n = 4 mice for each treatment group. h Integration frequency in 16-week post-transplant XIAP edited and AAV-transduced hCD45 cells by dPCR and flow cytometry. Flow cytometry data represent n = 1 per mouse, dPCR data shown as mean ± s.d. of n = 3 technical replicates. i CLEAR-time dPCR normalised ICE analysis of pre-transplant XIAP edited HSPCs and post-transplant XIAP edited hCD45 cells. All data represents n = 3 technical replicates unless stated otherwise. d – f Two-way ANOVA with Tukey post-hoc test. n.s.= no statistical significance, **** p < 0.0001. Source data are provided as a Source Data file.

Article Snippet: Human CD45-positive cells were positively enriched using hCD45 microbeads (Miltenyi Biotec) and MS columns (Miltenyi Biotec) following manufacturer instructions.

Techniques: Sequencing, Transduction, TALENs, Flow Cytometry

A Flow cytometry showing that few ALL cells (<1%) were expressing high level of HSP70. Immunostaining for HSP70 expression performed without permeabilization on BM cells from T-ALL and B-ALL PDX models. Flow cytometry is shown on hCD45 + cells for T-ALL and B-ALL viable cells (FVS450 negative). B Immunostaining on cells without permeabilization. Cells expressing HSP70 (HSP70 high) or negative for expression (HSP70 neg) were purified by FACS and observed by microscopy. EVs with membrane-anchored HSP70 expression were observed among the population of cells expressing HSP70. Example of microscopy for B-ALL cells. Microscopy with magnification ×63, scale bar represents 10 µm. C Procedure followed to isolate EVs in BM of PDX mice developing ALL. D Quantification and size of EVs isolated from BM with the NTA. Data are shown as means ± SD; n = 9 mice. P value measured by one-way Anova with Tukey’s multiple comparison test; **** P < 0.0001; ns, non-significant. E Procedure followed to isolate stain EVs prior to pull-down and analysis. F Flow cytometry confirming expression of HSP70 by EVs produced in T-ALL and B-ALL PDX mice, compared with control EVs produced by an NSG mouse. Data are representative of two independent experiments. G Microscopy on EVs purified from control NSG and ALL PDX models showing fluorescent EVs stained with HSP70-ATTO488, microscopy with magnification ×63, scale bar represents 5 µm.

Journal: Cell Death & Disease

Article Title: Acute lymphoblastic leukemia-derived extracellular vesicles affect quiescence of hematopoietic stem and progenitor cells

doi: 10.1038/s41419-022-04761-5

Figure Lengend Snippet: A Flow cytometry showing that few ALL cells (<1%) were expressing high level of HSP70. Immunostaining for HSP70 expression performed without permeabilization on BM cells from T-ALL and B-ALL PDX models. Flow cytometry is shown on hCD45 + cells for T-ALL and B-ALL viable cells (FVS450 negative). B Immunostaining on cells without permeabilization. Cells expressing HSP70 (HSP70 high) or negative for expression (HSP70 neg) were purified by FACS and observed by microscopy. EVs with membrane-anchored HSP70 expression were observed among the population of cells expressing HSP70. Example of microscopy for B-ALL cells. Microscopy with magnification ×63, scale bar represents 10 µm. C Procedure followed to isolate EVs in BM of PDX mice developing ALL. D Quantification and size of EVs isolated from BM with the NTA. Data are shown as means ± SD; n = 9 mice. P value measured by one-way Anova with Tukey’s multiple comparison test; **** P < 0.0001; ns, non-significant. E Procedure followed to isolate stain EVs prior to pull-down and analysis. F Flow cytometry confirming expression of HSP70 by EVs produced in T-ALL and B-ALL PDX mice, compared with control EVs produced by an NSG mouse. Data are representative of two independent experiments. G Microscopy on EVs purified from control NSG and ALL PDX models showing fluorescent EVs stained with HSP70-ATTO488, microscopy with magnification ×63, scale bar represents 5 µm.

Article Snippet: Lin − mouse BM cells (130-110-470, Miltenyi Biotec), c-Kit + mouse BM cells (130-091-224, Miltenyi Biotec), Sca1 + mouse BM cells (130-123-124, Miltenyi Biotec) or hCD45 + ALL cells (130-045-801, Miltenyi Biotec) were magnetically purified using LS columns (Miltenyi Biotec).

Techniques: Flow Cytometry, Expressing, Immunostaining, Purification, Microscopy, Membrane, Isolation, Comparison, Staining, Produced, Control